The creation of the championship was announced on August 20, 2009 during a backstage segment on TNA's primary television program TNA Impact!. Sarita and Taylor Wilde were the inaugural champions. They won a four-week tournament to crown the first champions. The final champions were ODB and Eric Young, who held the titles for a record 478 days.
On the June 20, 2013 episode of Impact Wrestling, Knockouts Division Executive Brooke Hogan stripped ODB and Young of the title because Young is a male talent, despite the fact that he had been a male for the prior 478 days. This ultimately resulted in the titles being deactivated, as it's no longer listed among TNA's active championships.
TV Air Dates Used where appropriate
- Sarita and Taylor Wilde: September 20, 2009 - TNA No Surrender 2009
- Awesome Kong and Hamada: January 4, 2010 - TNA Impact
Vacated — March 8, 2010
- The Beautiful People (Lacey Von Erich, Madison Rayne, and Velvet Sky): March 8, 2010 - TNA Impact
- Hamada and Taylor Wilde: August 5, 2010 - TNA Impact
Vacated — December 6, 2010
- Angelina Love and Winter: December 23, 2010 - TNA Impact
- Mexican America (Sarita and Rosita): March 13, 2011 - TNA Victory Road 2011
- TnT (Brooke Tessmacher and Tara): July 21, 2011 - Impact Wrestling
- Gail Kim and Madison Rayne: November 3, 2011 - Impact Wrestling
- Eric Young and ODB: March 8, 2012 - Impact Wrestling
Vacated — June 20, 2013 Deactivated — June 27, 2013